Isolation of B-1 subtype B lymphocytes from human cord blood
نویسندگان
چکیده
منابع مشابه
Isolation of Precursor B-cell Subsets from Umbilical Cord Blood
Umbilical cord blood is highly enriched for hematopoietic progenitor cells at different lineage commitment stages. We have developed a protocol for isolating precursor B-cells at four different stages of differentiation. Because genes are expressed and epigenetic modifications occur in a tissue specific manner, it is vital to discriminate between tissues and cell types in order to be able to id...
متن کاملPhenotype and Function of CD25-Expressing B Lymphocytes Isolated from Human Umbilical Cord Blood
BACKGROUND We have shown that approximately 30% of human peripheral blood B-cells express CD25. B cells expressing CD25 display a mature phenotype belonging to the memory B-cell population and have a better proliferative and antigen-presenting capacity. The aim of the present study was to characterize the CD25-expressing subset of B cells in human cord blood. MATERIAL AND METHODS Mononuclear ...
متن کاملIndividual CD34+CD38lowCD19-CD10- progenitor cells from human cord blood generate B lymphocytes and granulocytes.
Identification of human hematopoietic stem cells and analysis of molecular mechanisms regulating their function require biological assays that permit differentiation in all hematopoietic lineages simultaneously. In this study, we established conditions that permit the joint expression of the B-lymphoid and myeloid potential from cord blood-derived CD34+CD38lowCD19-/CD10- primitive progenitors t...
متن کاملB cell differentiation and immunoregulatory T cell function in human cord blood lymphocytes.
The functional maturity of T and B lymphocyte populations from human newborns was evaluated using a reverse hemolytic plaque assay to detect immunoglobulin-secreting cells generated in in vitro cultures stimulated with pokeweed mitogen (PWM), a T cell-dependent polyclonal activator, and the Epstein-Barr virus (EBV), a T cell-independent B cell activator. Cord blood lymphocytes failed to produce...
متن کاملSelective Expression of a VH1V Subfamily of Immunoglobulin Genes in Human CD5 + B Lymphocytes from Cord Blood
Human B lymphocytes expressing the CD5 surface antigen (CD5+ B cells) constitute a subset capable of producing polyspecific antibodies recognizing a variety of selfantigens . The repertoire of antibodies produced by CD5+ and CD5 B cells is different . However, it is not yet established whether this distribution is reflected in different immunoglobulin variable region gene (IgV) use. Rearrangeme...
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ژورنال
عنوان ژورنال: Pars of Jahrom University of Medical Sciences
سال: 2013
ISSN: 2008-7993,2008-8442
DOI: 10.29252/jmj.11.1.5